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dw2 wild type  (China Center for Type Culture Collection)

 
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    China Center for Type Culture Collection dw2 wild type
    AbrB negatively regulates pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of abrB deletion <t>(DW2</t> ΔabrB) and overexpression (DW2/pHY-abrB) on pulcherriminic acid production. (B) Gene transcription variations in abrB deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Binding of the AbrB protein to the promoter regions of yvmC and yvnA. Gel shifts of the labeled 91-bp P1-yvmC and P1-yvnA probes by the AbrB protein are shown. The concentrations of AbrB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P1-yvmC (lanes 1 to 4) or P1-yvnA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvnA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 and DW2 ΔabrB for the construction of DW2 ΔamyL and DW2 ΔabrB Δamy, respectively. pHY-PyvmC-amyL was then transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔabrB ΔamyL/pHY-PyvmC-amyL. pHY-PyvnA-amyL was transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvnA-amyL and DW2 ΔabrB ΔamyL/pHY-PyvnA-amyL.
    Dw2 Wild Type, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dw2+wild+type/dw2+wild+type/pmc06007100-18-0-4
    Average 90 stars, based on 1 article reviews
    dw2 wild type - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis"

    Article Title: Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00262-18

    AbrB negatively regulates pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of abrB deletion (DW2 ΔabrB) and overexpression (DW2/pHY-abrB) on pulcherriminic acid production. (B) Gene transcription variations in abrB deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Binding of the AbrB protein to the promoter regions of yvmC and yvnA. Gel shifts of the labeled 91-bp P1-yvmC and P1-yvnA probes by the AbrB protein are shown. The concentrations of AbrB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P1-yvmC (lanes 1 to 4) or P1-yvnA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvnA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 and DW2 ΔabrB for the construction of DW2 ΔamyL and DW2 ΔabrB Δamy, respectively. pHY-PyvmC-amyL was then transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔabrB ΔamyL/pHY-PyvmC-amyL. pHY-PyvnA-amyL was transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvnA-amyL and DW2 ΔabrB ΔamyL/pHY-PyvnA-amyL.
    Figure Legend Snippet: AbrB negatively regulates pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of abrB deletion (DW2 ΔabrB) and overexpression (DW2/pHY-abrB) on pulcherriminic acid production. (B) Gene transcription variations in abrB deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Binding of the AbrB protein to the promoter regions of yvmC and yvnA. Gel shifts of the labeled 91-bp P1-yvmC and P1-yvnA probes by the AbrB protein are shown. The concentrations of AbrB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P1-yvmC (lanes 1 to 4) or P1-yvnA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvnA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 and DW2 ΔabrB for the construction of DW2 ΔamyL and DW2 ΔabrB Δamy, respectively. pHY-PyvmC-amyL was then transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔabrB ΔamyL/pHY-PyvmC-amyL. pHY-PyvnA-amyL was transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvnA-amyL and DW2 ΔabrB ΔamyL/pHY-PyvnA-amyL.

    Techniques Used: Binding Assay, Over Expression, Labeling, Expressing, Transformation Assay, Construct

    YvmB represses pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of yvmB deletion (DW2 ΔyvmB) and overexpression (DW2/pHY-yvmB) on pulcherriminic acid production. (B) Gene transcription variations in yvmB deletion and overexpression strains. (C) Binding of the YvmB protein to the promoter regions of yvmC and yvmA. Gel shifts of the labeled P2-yvmC (87 bp) and P1-yvmA (91 bp) probes by the YvmB protein are shown. The concentrations of YvmB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P2-yvmC (lanes 1 to 4) or P1-yvmA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvmA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 ΔyvmB for the construction of DW2 ΔyvmB ΔamyL. pHY-PyvmC-amyL was transformed into DW2 ΔamyL and DW2 ΔyvmB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔyvmB ΔamyL/pHY-PyvmC-amyL. pHY-PyvmA-amyL was transformed into DW2 ΔamyL and DW2 ΔyvmB ΔamyL to construct DW2 ΔamyL/pHY-PyvmA-amyL and DW2 ΔyvmB ΔamyL/pHY-PyvmA-amyL.
    Figure Legend Snippet: YvmB represses pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of yvmB deletion (DW2 ΔyvmB) and overexpression (DW2/pHY-yvmB) on pulcherriminic acid production. (B) Gene transcription variations in yvmB deletion and overexpression strains. (C) Binding of the YvmB protein to the promoter regions of yvmC and yvmA. Gel shifts of the labeled P2-yvmC (87 bp) and P1-yvmA (91 bp) probes by the YvmB protein are shown. The concentrations of YvmB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P2-yvmC (lanes 1 to 4) or P1-yvmA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvmA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 ΔyvmB for the construction of DW2 ΔyvmB ΔamyL. pHY-PyvmC-amyL was transformed into DW2 ΔamyL and DW2 ΔyvmB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔyvmB ΔamyL/pHY-PyvmC-amyL. pHY-PyvmA-amyL was transformed into DW2 ΔamyL and DW2 ΔyvmB ΔamyL to construct DW2 ΔamyL/pHY-PyvmA-amyL and DW2 ΔyvmB ΔamyL/pHY-PyvmA-amyL.

    Techniques Used: Binding Assay, Over Expression, Labeling, Expressing, Transformation Assay, Construct

    Influence of YvnA on pulcherriminic acid biosynthesis. (A) Effects of yvnA on pulcherriminic acid production. The DW2 ΔyvnA ΔyvmB strain was set as a control to exclude the effects of yvmB. DW2/pHY-yvnA and DW2 ΔyvnA ΔyvmB/pHY-yvnA did not produce pulcherriminic acid (data not shown). (B) Effects of yvnA deletion and overexpression on gene transcription. (C) Binding of the YvnA protein to the promoter regions of yvmC and yvmB. Gel shifts of the labeled P2-yvmC (87 bp) and P2-yvmB (87 bp) probes by the YvnA protein are shown. The concentrations of YvnA in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P2-yvmC (lanes 1 to 4) or P2-yvmB (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvmB-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 ΔyvmB and DW2 ΔyvmB ΔyvnA for the construction of DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL, respectively. pHY-PyvmC-amyL was transformed into DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL to construct DW2 ΔyvmB ΔamyL/pHY-PyvmC-amyL and DW2 ΔyvmB ΔyvnA ΔamyL/pHY-PyvmC-amyL, respectively. pHY-PyvmB-amyL was transformed into DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL to construct DW2 ΔyvmB ΔamyL/pHY-PyvmB-amyL and DW2 ΔyvmB ΔyvnA ΔamyL/pHY-PyvmB-amyL, respectively.
    Figure Legend Snippet: Influence of YvnA on pulcherriminic acid biosynthesis. (A) Effects of yvnA on pulcherriminic acid production. The DW2 ΔyvnA ΔyvmB strain was set as a control to exclude the effects of yvmB. DW2/pHY-yvnA and DW2 ΔyvnA ΔyvmB/pHY-yvnA did not produce pulcherriminic acid (data not shown). (B) Effects of yvnA deletion and overexpression on gene transcription. (C) Binding of the YvnA protein to the promoter regions of yvmC and yvmB. Gel shifts of the labeled P2-yvmC (87 bp) and P2-yvmB (87 bp) probes by the YvnA protein are shown. The concentrations of YvnA in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P2-yvmC (lanes 1 to 4) or P2-yvmB (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvmB-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 ΔyvmB and DW2 ΔyvmB ΔyvnA for the construction of DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL, respectively. pHY-PyvmC-amyL was transformed into DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL to construct DW2 ΔyvmB ΔamyL/pHY-PyvmC-amyL and DW2 ΔyvmB ΔyvnA ΔamyL/pHY-PyvmC-amyL, respectively. pHY-PyvmB-amyL was transformed into DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL to construct DW2 ΔyvmB ΔamyL/pHY-PyvmB-amyL and DW2 ΔyvmB ΔyvnA ΔamyL/pHY-PyvmB-amyL, respectively.

    Techniques Used: Control, Over Expression, Binding Assay, Labeling, Expressing, Transformation Assay, Construct

    YvmA is required for secretion of pulcherriminic acid. (A) Effects of yvmA deletion (DW2 ΔyvmA) and overexpression (DW2/pHY-yvmA) on pulcherriminic acid production. (B) Gene transcription variations in yvmA deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Effects of yvmA deletion (DW2 ΔyvmA) and overexpression (DW2/pHY-yvmA) on intracellular accumulation of pulcherriminic acid.
    Figure Legend Snippet: YvmA is required for secretion of pulcherriminic acid. (A) Effects of yvmA deletion (DW2 ΔyvmA) and overexpression (DW2/pHY-yvmA) on pulcherriminic acid production. (B) Gene transcription variations in yvmA deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Effects of yvmA deletion (DW2 ΔyvmA) and overexpression (DW2/pHY-yvmA) on intracellular accumulation of pulcherriminic acid.

    Techniques Used: Over Expression

    Formation of pigmented halo and inhibition zone around F. oxysporum on PDA plates
    Figure Legend Snippet: Formation of pigmented halo and inhibition zone around F. oxysporum on PDA plates

    Techniques Used: Inhibition

    Effects of FeCl3 concentration on transcription of genes. The relative transcription levels of the genes in DW2 (A), DW2 ΔyvnA (B), and DW2 ΔyvnA ΔyvmB (C) in media containing 0, 5, and 20 mg/liter FeCl3 are shown. The transcription levels of genes in DW2 in the medium contain 0 mg/liter FeCl3 were set to 1.
    Figure Legend Snippet: Effects of FeCl3 concentration on transcription of genes. The relative transcription levels of the genes in DW2 (A), DW2 ΔyvnA (B), and DW2 ΔyvnA ΔyvmB (C) in media containing 0, 5, and 20 mg/liter FeCl3 are shown. The transcription levels of genes in DW2 in the medium contain 0 mg/liter FeCl3 were set to 1.

    Techniques Used: Concentration Assay

    Bacterial strains and plasmids used for this study
    Figure Legend Snippet: Bacterial strains and plasmids used for this study

    Techniques Used: Plasmid Preparation, Knock-Out, Expressing

    Related Articles

    Binding Assay:


    Over Expression:


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    Expressing:


    Transformation Assay:


    Construct:


    Control:


    Inhibition:


    Concentration Assay:


    Plasmid Preparation:


    Knock-Out:




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    dw2 wild type  (China Center for Type Culture Collection)
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    China Center for Type Culture Collection dw2 wild type
    AbrB negatively regulates pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of abrB deletion <t>(DW2</t> ΔabrB) and overexpression (DW2/pHY-abrB) on pulcherriminic acid production. (B) Gene transcription variations in abrB deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Binding of the AbrB protein to the promoter regions of yvmC and yvnA. Gel shifts of the labeled 91-bp P1-yvmC and P1-yvnA probes by the AbrB protein are shown. The concentrations of AbrB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P1-yvmC (lanes 1 to 4) or P1-yvnA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvnA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 and DW2 ΔabrB for the construction of DW2 ΔamyL and DW2 ΔabrB Δamy, respectively. pHY-PyvmC-amyL was then transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔabrB ΔamyL/pHY-PyvmC-amyL. pHY-PyvnA-amyL was transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvnA-amyL and DW2 ΔabrB ΔamyL/pHY-PyvnA-amyL.
    Dw2 Wild Type, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dw2+wild+type/dw2+wild+type/pmc06007100-18-0-4
    Average 90 stars, based on 1 article reviews
    dw2 wild type - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    AbrB negatively regulates pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of abrB deletion (DW2 ΔabrB) and overexpression (DW2/pHY-abrB) on pulcherriminic acid production. (B) Gene transcription variations in abrB deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Binding of the AbrB protein to the promoter regions of yvmC and yvnA. Gel shifts of the labeled 91-bp P1-yvmC and P1-yvnA probes by the AbrB protein are shown. The concentrations of AbrB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P1-yvmC (lanes 1 to 4) or P1-yvnA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvnA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 and DW2 ΔabrB for the construction of DW2 ΔamyL and DW2 ΔabrB Δamy, respectively. pHY-PyvmC-amyL was then transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔabrB ΔamyL/pHY-PyvmC-amyL. pHY-PyvnA-amyL was transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvnA-amyL and DW2 ΔabrB ΔamyL/pHY-PyvnA-amyL.

    Journal: Applied and Environmental Microbiology

    Article Title: Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis

    doi: 10.1128/AEM.00262-18

    Figure Lengend Snippet: AbrB negatively regulates pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of abrB deletion (DW2 ΔabrB) and overexpression (DW2/pHY-abrB) on pulcherriminic acid production. (B) Gene transcription variations in abrB deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Binding of the AbrB protein to the promoter regions of yvmC and yvnA. Gel shifts of the labeled 91-bp P1-yvmC and P1-yvnA probes by the AbrB protein are shown. The concentrations of AbrB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P1-yvmC (lanes 1 to 4) or P1-yvnA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvnA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 and DW2 ΔabrB for the construction of DW2 ΔamyL and DW2 ΔabrB Δamy, respectively. pHY-PyvmC-amyL was then transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔabrB ΔamyL/pHY-PyvmC-amyL. pHY-PyvnA-amyL was transformed into DW2 ΔamyL and DW2 ΔabrB ΔamyL to construct DW2 ΔamyL/pHY-PyvnA-amyL and DW2 ΔabrB ΔamyL/pHY-PyvnA-amyL.

    Article Snippet: DW2 , Wild type (CCTCC M2011344) , CCTCC.

    Techniques: Binding Assay, Over Expression, Labeling, Expressing, Transformation Assay, Construct

    YvmB represses pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of yvmB deletion (DW2 ΔyvmB) and overexpression (DW2/pHY-yvmB) on pulcherriminic acid production. (B) Gene transcription variations in yvmB deletion and overexpression strains. (C) Binding of the YvmB protein to the promoter regions of yvmC and yvmA. Gel shifts of the labeled P2-yvmC (87 bp) and P1-yvmA (91 bp) probes by the YvmB protein are shown. The concentrations of YvmB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P2-yvmC (lanes 1 to 4) or P1-yvmA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvmA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 ΔyvmB for the construction of DW2 ΔyvmB ΔamyL. pHY-PyvmC-amyL was transformed into DW2 ΔamyL and DW2 ΔyvmB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔyvmB ΔamyL/pHY-PyvmC-amyL. pHY-PyvmA-amyL was transformed into DW2 ΔamyL and DW2 ΔyvmB ΔamyL to construct DW2 ΔamyL/pHY-PyvmA-amyL and DW2 ΔyvmB ΔamyL/pHY-PyvmA-amyL.

    Journal: Applied and Environmental Microbiology

    Article Title: Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis

    doi: 10.1128/AEM.00262-18

    Figure Lengend Snippet: YvmB represses pulcherriminic acid biosynthesis by binding to the promoter of yvmC-cypX. (A) Effects of yvmB deletion (DW2 ΔyvmB) and overexpression (DW2/pHY-yvmB) on pulcherriminic acid production. (B) Gene transcription variations in yvmB deletion and overexpression strains. (C) Binding of the YvmB protein to the promoter regions of yvmC and yvmA. Gel shifts of the labeled P2-yvmC (87 bp) and P1-yvmA (91 bp) probes by the YvmB protein are shown. The concentrations of YvmB in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P2-yvmC (lanes 1 to 4) or P1-yvmA (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvmA-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 ΔyvmB for the construction of DW2 ΔyvmB ΔamyL. pHY-PyvmC-amyL was transformed into DW2 ΔamyL and DW2 ΔyvmB ΔamyL to construct DW2 ΔamyL/pHY-PyvmC-amyL and DW2 ΔyvmB ΔamyL/pHY-PyvmC-amyL. pHY-PyvmA-amyL was transformed into DW2 ΔamyL and DW2 ΔyvmB ΔamyL to construct DW2 ΔamyL/pHY-PyvmA-amyL and DW2 ΔyvmB ΔamyL/pHY-PyvmA-amyL.

    Article Snippet: DW2 , Wild type (CCTCC M2011344) , CCTCC.

    Techniques: Binding Assay, Over Expression, Labeling, Expressing, Transformation Assay, Construct

    Influence of YvnA on pulcherriminic acid biosynthesis. (A) Effects of yvnA on pulcherriminic acid production. The DW2 ΔyvnA ΔyvmB strain was set as a control to exclude the effects of yvmB. DW2/pHY-yvnA and DW2 ΔyvnA ΔyvmB/pHY-yvnA did not produce pulcherriminic acid (data not shown). (B) Effects of yvnA deletion and overexpression on gene transcription. (C) Binding of the YvnA protein to the promoter regions of yvmC and yvmB. Gel shifts of the labeled P2-yvmC (87 bp) and P2-yvmB (87 bp) probes by the YvnA protein are shown. The concentrations of YvnA in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P2-yvmC (lanes 1 to 4) or P2-yvmB (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvmB-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 ΔyvmB and DW2 ΔyvmB ΔyvnA for the construction of DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL, respectively. pHY-PyvmC-amyL was transformed into DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL to construct DW2 ΔyvmB ΔamyL/pHY-PyvmC-amyL and DW2 ΔyvmB ΔyvnA ΔamyL/pHY-PyvmC-amyL, respectively. pHY-PyvmB-amyL was transformed into DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL to construct DW2 ΔyvmB ΔamyL/pHY-PyvmB-amyL and DW2 ΔyvmB ΔyvnA ΔamyL/pHY-PyvmB-amyL, respectively.

    Journal: Applied and Environmental Microbiology

    Article Title: Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis

    doi: 10.1128/AEM.00262-18

    Figure Lengend Snippet: Influence of YvnA on pulcherriminic acid biosynthesis. (A) Effects of yvnA on pulcherriminic acid production. The DW2 ΔyvnA ΔyvmB strain was set as a control to exclude the effects of yvmB. DW2/pHY-yvnA and DW2 ΔyvnA ΔyvmB/pHY-yvnA did not produce pulcherriminic acid (data not shown). (B) Effects of yvnA deletion and overexpression on gene transcription. (C) Binding of the YvnA protein to the promoter regions of yvmC and yvmB. Gel shifts of the labeled P2-yvmC (87 bp) and P2-yvmB (87 bp) probes by the YvnA protein are shown. The concentrations of YvnA in lanes 1 to 8 were 0, 1, 2, 5, 0, 1, 2, and 5 ng/μl, respectively. Twenty nanograms of the P2-yvmC (lanes 1 to 4) or P2-yvmB (lanes 5 to 8) probe was added to each lane. The nonspecific competitor poly(dI-dC) was added to the EMSA binding buffer. (D) Expression-level comparisons of PyvmC-amyL and PyvmB-amyL transcriptional fusions. The amyL gene on the chromosome was deleted in DW2 ΔyvmB and DW2 ΔyvmB ΔyvnA for the construction of DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL, respectively. pHY-PyvmC-amyL was transformed into DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL to construct DW2 ΔyvmB ΔamyL/pHY-PyvmC-amyL and DW2 ΔyvmB ΔyvnA ΔamyL/pHY-PyvmC-amyL, respectively. pHY-PyvmB-amyL was transformed into DW2 ΔyvmB ΔamyL and DW2 ΔyvmB ΔyvnA ΔamyL to construct DW2 ΔyvmB ΔamyL/pHY-PyvmB-amyL and DW2 ΔyvmB ΔyvnA ΔamyL/pHY-PyvmB-amyL, respectively.

    Article Snippet: DW2 , Wild type (CCTCC M2011344) , CCTCC.

    Techniques: Control, Over Expression, Binding Assay, Labeling, Expressing, Transformation Assay, Construct

    YvmA is required for secretion of pulcherriminic acid. (A) Effects of yvmA deletion (DW2 ΔyvmA) and overexpression (DW2/pHY-yvmA) on pulcherriminic acid production. (B) Gene transcription variations in yvmA deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Effects of yvmA deletion (DW2 ΔyvmA) and overexpression (DW2/pHY-yvmA) on intracellular accumulation of pulcherriminic acid.

    Journal: Applied and Environmental Microbiology

    Article Title: Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis

    doi: 10.1128/AEM.00262-18

    Figure Lengend Snippet: YvmA is required for secretion of pulcherriminic acid. (A) Effects of yvmA deletion (DW2 ΔyvmA) and overexpression (DW2/pHY-yvmA) on pulcherriminic acid production. (B) Gene transcription variations in yvmA deletion and overexpression strains. The transcription levels of genes in DW2 were set to 1. (C) Effects of yvmA deletion (DW2 ΔyvmA) and overexpression (DW2/pHY-yvmA) on intracellular accumulation of pulcherriminic acid.

    Article Snippet: DW2 , Wild type (CCTCC M2011344) , CCTCC.

    Techniques: Over Expression

    Formation of pigmented halo and inhibition zone around F. oxysporum on PDA plates

    Journal: Applied and Environmental Microbiology

    Article Title: Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis

    doi: 10.1128/AEM.00262-18

    Figure Lengend Snippet: Formation of pigmented halo and inhibition zone around F. oxysporum on PDA plates

    Article Snippet: DW2 , Wild type (CCTCC M2011344) , CCTCC.

    Techniques: Inhibition

    Effects of FeCl3 concentration on transcription of genes. The relative transcription levels of the genes in DW2 (A), DW2 ΔyvnA (B), and DW2 ΔyvnA ΔyvmB (C) in media containing 0, 5, and 20 mg/liter FeCl3 are shown. The transcription levels of genes in DW2 in the medium contain 0 mg/liter FeCl3 were set to 1.

    Journal: Applied and Environmental Microbiology

    Article Title: Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis

    doi: 10.1128/AEM.00262-18

    Figure Lengend Snippet: Effects of FeCl3 concentration on transcription of genes. The relative transcription levels of the genes in DW2 (A), DW2 ΔyvnA (B), and DW2 ΔyvnA ΔyvmB (C) in media containing 0, 5, and 20 mg/liter FeCl3 are shown. The transcription levels of genes in DW2 in the medium contain 0 mg/liter FeCl3 were set to 1.

    Article Snippet: DW2 , Wild type (CCTCC M2011344) , CCTCC.

    Techniques: Concentration Assay

    Bacterial strains and plasmids used for this study

    Journal: Applied and Environmental Microbiology

    Article Title: Regulation of the Synthesis and Secretion of the Iron Chelator Cyclodipeptide Pulcherriminic Acid in Bacillus licheniformis

    doi: 10.1128/AEM.00262-18

    Figure Lengend Snippet: Bacterial strains and plasmids used for this study

    Article Snippet: DW2 , Wild type (CCTCC M2011344) , CCTCC.

    Techniques: Plasmid Preparation, Knock-Out, Expressing